Rapid Cycle Real-Time PCR — Methods and Applications

Rapid Cycle Real-Time PCR — Methods and Applications

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Rapid Cycle Real-Time PCR is a powerful analytical tool with broad application for the basic and applied life sciences. Compared with conventional PCR technology, Rapid Cycle Real-Time PCR is faster, has greater specificity, and is more easily adaptable for a variety of diagnostic tests, including qualitative, quantitative and mutation detection assays. This book provides general overviews of this technology for use in the clinical microbiology laboratory as well as specific diagnostic protocols for the detection of viral, bacterial and fungal pathogens and genetically modified organisms in human specimens and foodstuffs. All of these protocols have been developed, verified, and validated by experts in the field and should be of great interest for clinical microbiologists, pathologists, laboratory technologists as well as practicing physicians.The principles of nucleic acid extraction for commonly used conventional manual and automated and semiautomated methods are shown in Table 8. ... In this regard, development of analyte specific reagents (ASRs) or FDA-approved kits by vendors of real-time PCR is encouraged. ... membrane manual centrifugation steps ABIPRISM 6700 Applied Biosystems Proprietary Designed for integration withanbsp;...

Title:Rapid Cycle Real-Time PCR — Methods and Applications
Author: Udo Reischl, Carl Wittwer, F. Cockerill
Publisher:Springer Science & Business Media - 2012-12-06

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