Acceleration and Improvement of Protein Identification by Mass Spectrometry

Acceleration and Improvement of Protein Identification by Mass Spectrometry

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At present where protein identification and characterisation using mass spectrometry is a method of choice, this book is presenting a review of basic proteomic techniques. The second part of the book is related to the novel high throughput protein identification technique called the 'molecular scanner'. Several protein identification techniques are described, especially the peptide mass fingerprint with MALDI-MS based method. E.g. ionisation process, matrix available, signal reproducibility and suppression effect, as well as date treatment for protein identification using bioinformatics tools.Melanie 3 software (GeneBio, Geneva, CH) (24) was used for image treatment and band ... proteins separated by 1-DE was air dried between two cellophane sheets using an Easy Breeze Air Gel dryer (Hoefer, AP Biotech, Upsala, Sweden) .


Title:Acceleration and Improvement of Protein Identification by Mass Spectrometry
Author: Willy Vincent Bienvenut
Publisher:Springer Science & Business Media - 2005-04-19
ISBN-13:

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